Pseudouridination is a biochemical process that involves the conversion of the nucleoside uridine into pseudouridine. This modification occurs in RNA molecules and is important for their stability and function. Pseudouridine is often found in various types of RNA, including transfer RNA (tRNA) and ribosomal RNA (rRNA). This modification is catalyzed by specific enzymes known as pseudouridine synthases. Pseudouridination can enhance the structural integrity of RNA and influence its interactions with proteins and other molecules. It plays a crucial role in cellular processes, including protein synthesis and gene regulation.