Pseudouridine is a modified nucleoside found in various types of RNA, including transfer RNA (tRNA) and ribosomal RNA (rRNA). It is formed by the isomerization of uridine, where the nitrogen atom in the uracil base is replaced by a carbon atom, creating a more stable structure. This modification can enhance the stability and function of RNA molecules. Pseudouridine plays a crucial role in the proper folding and function of RNA. It helps maintain the structural integrity of RNA, which is essential for processes like protein synthesis and gene regulation. Researchers study pseudouridine to understand its implications in cellular functions and potential therapeutic applications.