Triose Phosphate Isomerase (TPI) is an enzyme that plays a crucial role in the glycolysis pathway, which is essential for energy production in cells. It catalyzes the reversible conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (G3P). This reaction helps maintain the balance of these two important molecules during carbohydrate metabolism. TPI is found in many organisms, including humans, and is located in the cytoplasm of cells. It is a homodimer, meaning it consists of two identical subunits. Deficiencies in TPI can lead to serious health issues, including hemolytic anemia and neurological problems, highlighting its importance in cellular function.