Traditional sequencing, also known as Sanger sequencing, is a method used to determine the precise order of nucleotides in a DNA molecule. Developed by Frederick Sanger in the 1970s, this technique involves amplifying the DNA sample and using labeled chain-terminating nucleotides to create fragments of varying lengths. These fragments are then separated by size through gel electrophoresis, allowing researchers to read the sequence. This method is highly accurate and has been widely used in various applications, including genome mapping and genetic testing. Although newer techniques like next-generation sequencing have emerged, traditional sequencing remains a reliable choice for smaller-scale projects and specific sequencing tasks.