Reverse Transcription PCR (RT-PCR) is a laboratory technique used to convert RNA into DNA. This process involves an enzyme called reverse transcriptase, which synthesizes complementary DNA (cDNA) from the RNA template. This cDNA can then be amplified using standard PCR methods, allowing researchers to study gene expression and detect specific RNA sequences. RT-PCR is widely used in various fields, including molecular biology, diagnostic medicine, and virology. It is particularly valuable for detecting viral infections, such as COVID-19, by identifying the presence of viral RNA in patient samples. This technique provides insights into gene activity and helps in understanding disease mechanisms.