Real-Time PCR, or quantitative PCR, is a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule. It allows researchers to monitor the progress of the PCR reaction in real-time, providing immediate feedback on the amount of DNA present. This is achieved through the use of fluorescent dyes that emit light when bound to the DNA, enabling precise measurement of DNA concentration during each cycle of amplification. This method is widely used in various fields, including molecular biology, genetics, and clinical diagnostics. It is particularly valuable for applications such as detecting pathogens, measuring gene expression, and genotyping, making it an essential tool in modern scientific research.