RNA-Seq is a powerful technique used to analyze the complete set of RNA molecules in a cell or tissue at a specific time. It allows researchers to measure gene expression levels, identify new genes, and discover alternative splicing events. By converting RNA into complementary DNA (cDNA) and sequencing it, scientists can obtain a detailed snapshot of the active genes in a sample. This method has revolutionized the field of genomics, providing insights into various biological processes and diseases. RNA-Seq is widely used in research areas such as cancer, developmental biology, and microbiology, helping to advance our understanding of complex biological systems.