Immunoblotting, also known as Western blotting, is a laboratory technique used to detect specific proteins in a sample. The process begins with separating proteins by size using gel electrophoresis, followed by transferring them onto a membrane. This allows for easier access to the proteins for further analysis. After transfer, the membrane is incubated with antibodies that specifically bind to the target protein. These antibodies are often linked to a detectable marker, enabling visualization of the protein. This method is widely used in research and clinical diagnostics to study protein expression and function.