Western blotting is a laboratory technique used to detect specific proteins in a sample. The process begins with separating proteins by size using gel electrophoresis, followed by transferring them onto a membrane. This allows for easier identification and analysis of the proteins. After transfer, the membrane is treated with antibodies that specifically bind to the target protein. These antibodies are often linked to a detectable marker, enabling visualization. The intensity of the signal indicates the amount of the protein present, making Western blotting a valuable tool in research and diagnostics for studying protein expression and function.