Western Blot is a laboratory technique used to detect specific proteins in a sample. It involves separating proteins by size using gel electrophoresis, transferring them to a membrane, and then probing with antibodies that bind to the target protein. This allows researchers to identify and quantify proteins of interest. The process typically includes several steps: sample preparation, gel electrophoresis, transfer to a membrane, blocking non-specific binding sites, and incubation with primary and secondary antibodies. Finally, a detection method reveals the presence of the target protein, making Western Blot a valuable tool in molecular biology and medical research.