High-Throughput Sequencing (HTS) is a modern technique that allows scientists to quickly and efficiently determine the sequence of DNA or RNA. Unlike traditional sequencing methods, which can be slow and labor-intensive, HTS can process millions of DNA fragments simultaneously. This capability makes it possible to analyze entire genomes or transcriptomes in a fraction of the time. HTS has revolutionized fields such as genomics, transcriptomics, and metagenomics, enabling researchers to explore genetic variations, study gene expression, and investigate complex microbial communities. The data generated from HTS can lead to significant advancements in areas like personalized medicine and evolutionary biology.