Fructose 1,6-Bisphosphate is a key intermediate in the metabolic pathway of glycolysis, which is the process that breaks down glucose to produce energy. It is formed from fructose 6-phosphate through the action of the enzyme phosphofructokinase, and it plays a crucial role in regulating the flow of carbon through the glycolytic pathway. This compound is a six-carbon sugar phosphate that can be further split into two three-carbon molecules, glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. These molecules continue through glycolysis, ultimately leading to the production of ATP, the energy currency of the cell.