Direct inoculation is a method used in microbiology to introduce a specific microorganism directly into a culture medium. This technique allows researchers to grow and study the organism in a controlled environment, facilitating the observation of its characteristics and behaviors. It is commonly used for bacteria, fungi, and other microorganisms. This process often involves using a sterile tool, such as an inoculating loop, to transfer a small sample from a source, like a petri dish or broth culture, to a new medium. Direct inoculation is essential for isolating pure cultures and conducting experiments in fields like medicine and agriculture.