A broth culture is a method used in microbiology to grow and maintain microorganisms, such as bacteria or yeast, in a liquid nutrient medium. This technique allows for the multiplication of cells in a controlled environment, making it easier to study their characteristics and behaviors. The broth typically contains essential nutrients, such as sugars, amino acids, and vitamins, to support the growth of the microorganisms. In a broth culture, the microorganisms are usually inoculated into the liquid medium and incubated under specific conditions, such as temperature and pH. This method is commonly used in laboratories for various applications, including research, diagnostics, and the production of biological products like antibiotics. Proper handling and sterilization are crucial to prevent contamination from unwanted organisms.