The Battle of Agnadello took place on May 14, 1509, during the Italian Wars. It was fought between the forces of the Duchy of Milan, led by Francesco Sforza, and the Venetian Republic. The battle was significant as it marked a decisive victory for the Milanese, which allowed them to gain control over much of northern Italy. The conflict arose from territorial disputes and power struggles among various Italian states. The defeat of the Venetians at Agnadello weakened their influence and led to the loss of several territories, reshaping the political landscape of Italy during this period.