Ultraviolet-visible spectroscopy, often abbreviated as UV-Vis spectroscopy, is an analytical technique used to measure the absorbance of light in the ultraviolet and visible regions of the electromagnetic spectrum. This method helps identify and quantify substances based on how they absorb light at specific wavelengths. It is widely used in chemistry, biology, and environmental science. In UV-Vis spectroscopy, a sample is exposed to light, and the amount of light absorbed is recorded. The resulting data can provide insights into the electronic structure of molecules, making it useful for studying compounds like proteins, nucleic acids, and various chemical substances.