Single-cell RNA sequencing (scRNA-seq) is a powerful technique used to analyze the gene expression of individual cells. Unlike traditional RNA sequencing, which averages the gene expression across many cells, scRNA-seq allows researchers to capture the unique molecular profiles of each cell. This helps in understanding cellular diversity and the roles of different cell types in various biological processes. The process involves isolating single cells, extracting their RNA, and then sequencing it to identify which genes are active. This information can reveal insights into cellular functions, developmental stages, and responses to treatments, making scRNA-seq a valuable tool in genomics and biomedical research.