The mitotic spindle is a structure made of microtubules that forms during cell division, specifically during mitosis. It plays a crucial role in separating duplicated chromosomes into two daughter cells. The spindle fibers attach to the chromosomes at their centromeres, ensuring that each new cell receives the correct number of chromosomes. During metaphase, the mitotic spindle aligns the chromosomes at the cell's equator, preparing them for separation. Once aligned, the spindle fibers pull the sister chromatids apart during anaphase, guiding them to opposite poles of the cell. This process is essential for maintaining genetic stability in the resulting daughter cells.