Enolase is an enzyme that plays a crucial role in the glycolysis pathway, which is the process by which cells convert glucose into energy. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, facilitating the production of ATP, the energy currency of the cell. Enolase is found in various organisms, including bacteria, plants, and animals. This enzyme exists in several isoforms, with alpha-enolase and beta-enolase being the most studied in humans. Enolase is also involved in other cellular processes, such as the regulation of gene expression and cell signaling. Its activity can be influenced by factors like pH and the presence of metal ions.