Beta-enolase is an enzyme that plays a crucial role in the glycolysis pathway, which is the process by which cells convert glucose into energy. Specifically, beta-enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, facilitating the production of ATP, the energy currency of the cell. This enzyme is primarily found in muscle and brain tissues, where energy demand is high. Beta-enolase is one of the three enolase isoforms, with the other two being alpha-enolase and gamma-enolase. Each isoform has distinct functions and tissue distributions, contributing to the overall metabolic processes in the body.