Biological safety cabinets (BSCs) are specialized workspaces designed to protect users, the environment, and samples from contamination. They provide a sterile environment by using high-efficiency particulate air (HEPA) filters to trap harmful microorganisms and particles. BSCs are commonly used in laboratories that handle pathogens, biological materials, or toxic substances. There are different classes of BSCs, each suited for specific applications. For example, Class I cabinets offer personnel protection but do not protect the samples, while Class II cabinets provide both personnel and product protection. Proper use and maintenance of BSCs are essential for ensuring safety and compliance with biosafety regulations.