Autoclaving is a sterilization process that uses high-pressure steam to eliminate bacteria, viruses, and other microorganisms from various materials. This method is commonly used in medical settings to sterilize surgical instruments, laboratory equipment, and other items that require a high level of cleanliness. The autoclave operates at temperatures typically around 121°C (250°F) for a specific duration, ensuring effective sterilization. The process involves placing items inside a sealed chamber, where steam is introduced and pressure is increased. This combination of heat and pressure penetrates materials, ensuring that even hard-to-reach areas are sterilized. Autoclaving is a reliable and widely accepted method for maintaining hygiene in healthcare and laboratory environments.