Tryptic Soy Broth (TSB) is a nutrient-rich liquid medium used in microbiology for the growth of a wide variety of bacteria and fungi. It contains peptones derived from soybean and casein, which provide essential amino acids and nitrogen sources. TSB is often used for cultivating microorganisms in laboratory settings, making it a staple in research and clinical diagnostics. This broth is particularly useful for growing fastidious organisms that require specific nutrients. It supports the growth of both aerobic and anaerobic bacteria, making it versatile for various applications, including antibiotic susceptibility testing and biochemical assays.