Single-molecule manipulation is a technique used in scientific research to study individual molecules rather than bulk samples. This approach allows scientists to observe the behavior and properties of single molecules, providing insights into their functions and interactions. Techniques such as atomic force microscopy (AFM) and optical tweezers are commonly employed to grasp and move these molecules with precision. This method is crucial in fields like biophysics and nanotechnology, where understanding the dynamics of single molecules can lead to advancements in drug development and materials science. By isolating and manipulating single molecules, researchers can explore fundamental processes at the molecular level, enhancing our knowledge of complex biological systems.