A replication fork is a Y-shaped structure that forms during the process of DNA replication. It occurs when the double-stranded DNA molecule unwinds and separates into two single strands, allowing each strand to serve as a template for creating new complementary strands. This process is essential for cell division and ensures that genetic information is accurately copied and passed on to daughter cells. At the replication fork, several key enzymes work together to facilitate DNA synthesis. For example, the enzyme DNA polymerase adds nucleotides to the growing DNA strands, while helicase unwinds the DNA helix. Additionally, single-strand binding proteins stabilize the unwound strands, preventing them from re-annealing before replication is complete.