RT-PCR, or Reverse Transcription Polymerase Chain Reaction, is a laboratory technique used to detect and quantify RNA. It involves converting RNA into complementary DNA (cDNA) using an enzyme called reverse transcriptase. This cDNA can then be amplified through the polymerase chain reaction, allowing scientists to analyze specific RNA sequences. This method is widely used in various fields, including molecular biology, genetics, and medical diagnostics. RT-PCR is particularly important for studying gene expression and identifying viral infections, such as those caused by SARS-CoV-2, the virus responsible for COVID-19.