Phase Contrast Microscopy is a technique that enhances the contrast of transparent and colorless specimens, making them easier to observe under a microscope. It works by converting phase shifts in light passing through the specimen into variations in brightness. This allows scientists to view live cells and other delicate structures without staining, preserving their natural state. This method is particularly useful in biological research, as it enables the study of cellular processes in real-time. Phase Contrast Microscopy is widely used in fields such as microbiology, cytology, and histology, providing valuable insights into the behavior and characteristics of living organisms.