Differential Interference Contrast (DIC) is a microscopy technique that enhances the contrast of transparent specimens. It uses polarized light and a special optical setup to create a three-dimensional appearance of the sample. This method is particularly useful for observing live cells and biological tissues, as it reveals details without the need for staining. DIC works by exploiting differences in the refractive index of various parts of the specimen. When light passes through the sample, it is bent differently depending on the material's properties. This variation in light paths is converted into contrast, allowing for clearer visualization of fine structures within the sample.