Phase Contrast is a microscopy technique that enhances the visibility of transparent specimens, such as living cells, by converting phase shifts in light waves into variations in brightness. This allows scientists to observe details that are otherwise difficult to see with standard light microscopy. The method works by using a special optical setup that includes a phase plate and annular diaphragm. These components manipulate the light passing through the specimen, making it possible to visualize structures like cell membranes and organelles without the need for staining, which can alter or damage the sample.