New York State Route 400 is a north-south highway located in western New York. It runs approximately 18 miles, connecting the city of Buffalo to Interstate 90, providing access to various local communities and attractions along the way. The route begins at New York State Route 5 in Buffalo and extends to New York State Route 16 in Elma. It features a mix of urban and suburban landscapes, making it an important thoroughfare for both commuters and travelers in the region.