The mitotic spindle is a structure made of microtubules that forms during cell division, specifically during the process of mitosis. It plays a crucial role in ensuring that chromosomes are accurately separated into two daughter cells. The spindle fibers attach to the chromosomes at their centromeres, helping to align and pull them apart. During metaphase, the mitotic spindle aligns the chromosomes at the cell's equatorial plane. As the cell progresses to anaphase, the spindle fibers shorten, pulling the sister chromatids toward opposite poles of the cell. This ensures that each new cell receives the correct number of chromosomes, maintaining genetic stability.