The MTT Assay is a colorimetric method used to assess cell viability and proliferation. It involves the reduction of the yellow MTT dye to purple formazan crystals by metabolically active cells. The intensity of the purple color, measured using a spectrophotometer, correlates with the number of viable cells. This assay is widely used in biomedical research and drug development to evaluate the effects of treatments on cell health. It is simple, cost-effective, and allows for high-throughput screening, making it a popular choice for researchers studying cell behavior and responses to various stimuli.