HTSlib is an open-source software library designed for reading and writing high-throughput sequencing data. It supports various file formats, including BAM, SAM, and CRAM, which are commonly used in genomics for storing sequence alignment data. The library is widely used in bioinformatics for its efficiency and ability to handle large datasets. Developed as part of the HTS (High-Throughput Sequencing) project, HTSlib provides essential tools for researchers working with genomic data. It is often integrated into other bioinformatics tools and pipelines, making it a fundamental resource for analyzing sequencing results and facilitating data sharing in the scientific community.