The Genome Analysis Toolkit (GATK) is a software package developed by the Broad Institute for analyzing high-throughput sequencing data. It provides tools for variant discovery, genotyping, and data processing, making it essential for researchers in genomics. GATK is widely used in studies related to human genetics, cancer research, and population genomics. GATK employs a best-practice framework that guides users through the analysis pipeline, ensuring high-quality results. It supports various sequencing technologies and formats, allowing for flexibility in research applications. The toolkit is continuously updated to incorporate new methods and improvements, reflecting advancements in the field of genomics.