ChIP-Seq, or Chromatin Immunoprecipitation Sequencing, is a powerful technique used to study the interactions between proteins and DNA in cells. It combines chromatin immunoprecipitation, which isolates specific protein-DNA complexes, with next-generation sequencing to identify the binding sites of proteins across the genome. This helps researchers understand gene regulation and the role of various proteins in cellular processes. The process begins with cross-linking proteins to DNA, followed by fragmentation and immunoprecipitation using antibodies specific to the protein of interest. After sequencing the isolated DNA, bioinformatics tools analyze the data to map the binding sites, providing insights into how proteins influence gene expression and cellular function.