BrdU incorporation is a technique used to study cell proliferation by tracking the incorporation of bromodeoxyuridine (BrdU), a synthetic nucleoside, into newly synthesized DNA. When cells divide, they replicate their DNA, and BrdU can be incorporated in place of thymidine, allowing researchers to identify actively dividing cells. After treatment with BrdU, cells are typically fixed and stained using specific antibodies that bind to BrdU. This allows scientists to visualize and quantify the number of cells that have incorporated BrdU, providing insights into cell growth, development, and the effects of various treatments on cell division.