Aldolases are enzymes that play a crucial role in the metabolism of carbohydrates. They catalyze the reversible reaction that combines two molecules of sugar, specifically fructose and glyceraldehyde-3-phosphate, to form a larger sugar molecule called fructose-1,6-bisphosphate. This process is essential in both the glycolysis and gluconeogenesis pathways, which are vital for energy production in cells. There are different types of aldolases, including aldolase A, aldolase B, and aldolase C, each found in various tissues and having specific functions. For example, aldolase A is primarily present in muscle and brain tissues, while aldolase B is found in the liver. These enzymes help regulate sugar metabolism, ensuring that cells have the energy they need to function properly.