Affinity Chromatography is a powerful technique used to purify proteins, enzymes, or other biomolecules based on their specific interactions with a ligand. In this method, a column is packed with a stationary phase that contains a ligand attached to a solid support. When a mixture is passed through the column, only the target molecule binds to the ligand, while other components are washed away. After the unbound substances are removed, the target molecule can be eluted by changing the conditions, such as altering the pH or adding a competing substance. This selective binding and elution make Affinity Chromatography highly effective for isolating specific biomolecules from complex mixtures.