3D Microscopy is an advanced imaging technique that allows scientists to visualize samples in three dimensions. Unlike traditional microscopy, which provides only flat images, 3D microscopy captures multiple layers of data, enabling a more detailed view of the structure and organization of biological specimens, materials, or other objects. This technique often employs methods like confocal microscopy or two-photon microscopy to create high-resolution images. By reconstructing these images digitally, researchers can analyze complex structures, study cellular interactions, and gain insights into various scientific fields, including biology, materials science, and medicine.