Confocal microscopy is an advanced imaging technique that enhances the clarity of images obtained from biological samples. It uses a laser to illuminate a specific point in the sample and collects emitted light through a pinhole aperture, which helps eliminate out-of-focus light. This results in high-resolution, three-dimensional images of cells and tissues. This method is particularly useful in cell biology and neuroscience, allowing researchers to visualize structures at a microscopic level. By capturing images at different depths, confocal microscopy enables the reconstruction of detailed 3D models, providing valuable insights into the organization and function of complex biological systems.