Tryptic Soy Agar (TSA) is a nutrient-rich medium used in microbiology to grow a wide variety of bacteria and fungi. It contains peptones, which provide essential amino acids and nitrogen, and agar, a gelatinous substance that solidifies the medium. TSA supports the growth of both fastidious and non-fastidious organisms, making it a versatile choice for laboratory cultures. TSA is commonly used for isolating and counting microorganisms from various samples, including food, water, and clinical specimens. Its balanced formulation allows researchers to study microbial characteristics, perform antibiotic susceptibility tests, and conduct other microbiological analyses effectively.