trypan blue exclusion
Trypan blue exclusion is a method used to determine cell viability in biological research. In this technique, trypan blue, a blue dye, is added to a cell suspension. Live cells exclude the dye and remain clear, while dead cells take up the dye and appear blue under a microscope.
This simple staining method allows researchers to quickly assess the health of cells in culture. By counting the number of blue (dead) and clear (live) cells, scientists can calculate the percentage of viable cells, providing valuable information about cell growth and the effects of treatments.