super-resolution microscopy
Super-resolution microscopy is an advanced imaging technique that allows scientists to visualize structures at a resolution beyond the diffraction limit of light. Traditional light microscopy can only resolve details down to about 200 nanometers, but super-resolution methods can achieve resolutions of 20 nanometers or even less. This enhanced clarity enables researchers to study cellular components and interactions in unprecedented detail.
One popular super-resolution technique is STORM (Stochastic Optical Reconstruction Microscopy), which uses fluorescent molecules that turn on and off to create high-resolution images. Another method, PALM (Photo-Activated Localization Microscopy), relies on similar principles. These techniques have revolutionized fields like cell biology and neuroscience, providing insights into complex biological processes.