Isothermal titration calorimetry (ITC) is a technique used to measure the heat change that occurs during a chemical reaction, particularly in binding interactions. It provides insights into the thermodynamics of molecular interactions by determining parameters such as binding affinity, stoichiometry, and enthalpy changes. In an ITC experiment, a sample solution is titrated with a ligand while monitoring the heat released or absorbed. The data collected allows researchers to construct binding curves, which help in understanding the strength and nature of interactions between biomolecules, such as proteins and ligands.