Glucose-6-phosphatase is an enzyme primarily found in the liver and kidneys. It plays a crucial role in glucose metabolism by converting glucose-6-phosphate into free glucose and phosphate. This process is essential for maintaining blood sugar levels, especially during fasting or intense exercise. The enzyme is part of the gluconeogenesis and glycogenolysis pathways, which help the body produce and release glucose when needed. Deficiencies in glucose-6-phosphatase can lead to metabolic disorders, such as Glycogen Storage Disease type I, which can cause severe hypoglycemia and other health issues.