Cytosolic aconitase is an enzyme found in the cytosol of cells, playing a crucial role in the citric acid cycle, also known as the Krebs cycle. It catalyzes the conversion of citrate to isocitrate, facilitating energy production. This process is essential for cellular metabolism and energy generation. In addition to its enzymatic function, cytosolic aconitase also acts as an iron-sensing protein. It can bind to iron and regulate the expression of genes involved in iron metabolism. This dual role highlights its importance in both energy production and maintaining cellular iron homeostasis.