Ultraviolet-Visible Spectroscopy
Ultraviolet-Visible Spectroscopy (UV-Vis) is an analytical technique used to measure the absorbance of light by a sample in the ultraviolet and visible regions of the electromagnetic spectrum. This method helps identify and quantify substances based on their unique absorption characteristics, which are influenced by their molecular structure.
In a typical UV-Vis experiment, a light source emits radiation that passes through a sample. The amount of light absorbed at specific wavelengths is recorded, creating a spectrum. This data can be used to determine the concentration of a compound, monitor chemical reactions, and study the properties of materials.