Two-Dimensional Gel Electrophoresis (2DGE) is a laboratory technique used to separate proteins based on their isoelectric point and molecular weight. In the first dimension, proteins are separated in a gel using an electric field that causes them to migrate until they reach a pH where they have no net charge. In the second dimension, the proteins are further separated by size using a different gel. This method allows researchers to analyze complex protein mixtures, making it valuable for studies in proteomics and biomedical research. 2DGE helps identify and quantify proteins, aiding in the understanding of various biological processes.