Topoisomerase I is an essential enzyme that helps manage the structure of DNA during processes like replication and transcription. It works by creating temporary breaks in one strand of the DNA double helix, allowing the strands to unwind and relieve tension caused by supercoiling. After the tension is released, the enzyme reseals the break, restoring the DNA to its original state. This enzyme is crucial for maintaining DNA integrity and ensuring proper cellular function. Inhibitors of Topoisomerase I are also used in cancer treatments, as they can disrupt the replication of cancer cells by preventing proper DNA unwinding and rejoining.