T-Coffee is a software tool used in bioinformatics for multiple sequence alignment. It helps researchers compare and align sequences of DNA, RNA, or proteins, making it easier to identify similarities and differences among them. This is crucial for understanding evolutionary relationships and functional characteristics. The name T-Coffee stands for "Tree-based Consistency Objective Function For alignment Evaluation." It uses a unique approach that combines different alignment methods to improve accuracy. By integrating various data sources, T-Coffee provides a more reliable alignment, which is essential for studies in genetics, molecular biology, and evolutionary biology.